plant expression vector pmdc32 hpb (Addgene inc)
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Plant Expression Vector Pmdc32 Hpb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmdc32+hpb/pMDC32-HPB+(Plasmid+%2332078)/bio_rxiv__2025__05__06__652549-219-13-18
Average 93 stars, based on 13 article reviews
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other:Article Title: Differences in PpAAT1 Activity in High- and Low-Aroma Peach Varieties Affect γ -Decalactone Production Article Snippet: For the MS/MS detection, all precursors were fragmented using 20 to 40 eV, and the scan time was 0.2 s. The MS data were analyzed using the Progenesis QI program (version 2.2; Waters). Expressing:Article Title: Differences in PpAAT1 Activity in High- and Low-Aroma Peach Varieties Affect γ -Decalactone Production Article Snippet: A constant flow rate of 0.4 mL min −1 was used with the following gradient elution conditions: initially 100% A, held for 0 to 2 min; then 2 to 9 min 0% B to 100% B; and then 100% B, held for 9 to 11 min. A high-resolution tandem mass spectrometer (Xevo G2-XS QTOF; Waters) was used to detect the metabolites eluted from the column. .. Transient Expression in Nicotiana benthamiana The PpAAT1 cDNA clone was transferred into the Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches. Plasmid Preparation:Article Title: Differences in PpAAT1 Activity in High- and Low-Aroma Peach Varieties Affect γ -Decalactone Production Article Snippet: A constant flow rate of 0.4 mL min −1 was used with the following gradient elution conditions: initially 100% A, held for 0 to 2 min; then 2 to 9 min 0% B to 100% B; and then 100% B, held for 9 to 11 min. A high-resolution tandem mass spectrometer (Xevo G2-XS QTOF; Waters) was used to detect the metabolites eluted from the column. .. Transient Expression in Nicotiana benthamiana The PpAAT1 cDNA clone was transferred into the Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape Article Snippet: To evaluate the enzymatic activity of VvCYP76F14 in berries, the CDS of VvCYP76F14 was isolated from ‘L35’, ‘Merlot’, or D299T mutant lines, respectively, and synthesized by GenScript Co., Ltd. (Nanjing, China). .. Sequencing-verified VvCYP76F14 was cloned into the Transformation Assay:Article Title: Differences in PpAAT1 Activity in High- and Low-Aroma Peach Varieties Affect γ -Decalactone Production Article Snippet: A constant flow rate of 0.4 mL min −1 was used with the following gradient elution conditions: initially 100% A, held for 0 to 2 min; then 2 to 9 min 0% B to 100% B; and then 100% B, held for 9 to 11 min. A high-resolution tandem mass spectrometer (Xevo G2-XS QTOF; Waters) was used to detect the metabolites eluted from the column. .. Transient Expression in Nicotiana benthamiana The PpAAT1 cDNA clone was transferred into the Activity Assay:Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Isolation:Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Mutagenesis:Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Synthesized:Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Clone Assay:Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape Article Snippet: To evaluate the enzymatic activity of VvCYP76F14 in berries, the CDS of VvCYP76F14 was isolated from ‘L35’, ‘Merlot’, or D299T mutant lines, respectively, and synthesized by GenScript Co., Ltd. (Nanjing, China). .. Sequencing-verified VvCYP76F14 was cloned into the Virus:Article Title: Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits Article Snippet: The turnover number ( k cat ) and affinity ( K m ) were calculated using Origin 2018 ( www.originlab.com ). .. To assess the enzymatic activity of PpAAT1 and PpAAT1-SMs in plants, PpAAT1 isolated from ‘Fenghuayulu’ and its mutant forms ( PpAAT1 - SMs ) synthesized at GenScript Co. Ltd. (Nanjing, China) were cloned into Amplification:Article Title: Potential role of fatty acid desaturase 2 in regulating peach aroma formation Article Snippet: Fruit aroma is an important evaluation index for fruit quality.. Linoleic acid is an essential precursor pool for the aroma compounds in peach (Prunus persica L.) and is catalyzed by fatty acid desaturase 2 (FAD2).. In this study, PpFAD2–1 and PpFAD2–2 were isolated from ‘Jinxiuhuangtao’ peaches. Sequencing:Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape Article Snippet: To evaluate the enzymatic activity of VvCYP76F14 in berries, the CDS of VvCYP76F14 was isolated from ‘L35’, ‘Merlot’, or D299T mutant lines, respectively, and synthesized by GenScript Co., Ltd. (Nanjing, China). .. Sequencing-verified VvCYP76F14 was cloned into the Recombinant:Article Title: D299T Mutation in CYP76F14 Led to a Decrease in Wine Bouquet Precursor Production in Wine Grape Article Snippet: To evaluate the enzymatic activity of VvCYP76F14 in berries, the CDS of VvCYP76F14 was isolated from ‘L35’, ‘Merlot’, or D299T mutant lines, respectively, and synthesized by GenScript Co., Ltd. (Nanjing, China). .. Sequencing-verified VvCYP76F14 was cloned into the |
